Date: 12/09/2009 
Attendance: 530
Competition: National League / North
Referee: Ian Dudley
 Gainsborough Trinity |
2 - 4 Full-Time |
 Southport |
| Manager: Liam Watson | ||
| Goalscorers | ||
 Ciaran Kilheeney (13') Matty McGinn (39') Steve Daly (61', 70') |
||
Line-Ups
Substitutes
| Chris Lever | |
| Zac Aley (  Matty McGinn 73) | |
| Bradley Barnes ( Paul Barratt 72) | |
| Steve Daly (61', 70') ( Ashley Winn 51) | |
| Shaun Gray |